Journal: International Journal of Molecular Sciences

Article Title: MAPK Phosphatase-3 Mediates Chronic Endoplasmic Reticulum Stress Promoting Hepatic Gluconeogenesis

doi: 10.3390/ijms27062874

Figure Lengend Snippet: Chronic ER stress promoted gluconeogenesis and Mkp-3 expression. ( A ) Primary mouse hepatocytes were treated with Tg (2 ng/mL) for 10 days or cultured with PA (50 µM) for 4 days, or with their respective controls. ( B ) Expression levels of ER stress marker genes Grp78 , Chop and Xbp1 in Tg-treated hepatocytes ( n = 3). ( C ) Expression levels of gluconeogenic genes Pepck and G6pc , and their regulator Pgc1α in Tg-treated hepatocytes ( n = 3). ( D ) Expression level of Mkp-3 in Tg-treated hepatocytes ( n = 3). ( E ) Expression of ER stress marker genes Grp78 , Chop and Xbp1 in PA-treated hepatocytes ( n = 3). ( F ) Expression level of Mkp-3 in PA-treated hepatocytes ( n = 3). Data are presented as mean ± standard error. * p < 0.05, ** p < 0.01, *** p < 0.001, ns indicates no significant difference.

Article Snippet: Briefly, Mkp-3 loxp/loxp mice (S-CKO-13779, Cyagen Biosciences, Guangzhou, China) were mated with albumin-Cre mice (Jackson Laboratory, Bar Harbor, ME, USA) to generate Mkp-3 LKO ( Mkp-3 loxp/loxp , Cre + ) mice.

Techniques: Expressing, Cell Culture, Marker

Journal: International Journal of Molecular Sciences

Article Title: MAPK Phosphatase-3 Mediates Chronic Endoplasmic Reticulum Stress Promoting Hepatic Gluconeogenesis

doi: 10.3390/ijms27062874

Figure Lengend Snippet: Chronic Tg treatment induced ER stress in mouse liver and promoted gluconeogenesis and Mkp-3 expression. ( A ) Mice were intraperitoneally injected with Tg (0.075 mg/kg body weight) or equivalent volume of DMSO/saline solution for 35 days. ( B ) Changes in body weight during the treatment ( n = 6). ( C ) Liver weight ( n = 6). ( D ) Fasting blood glucose concentrations at harvest time ( n = 6). ( E ) Expression levels of ER stress marker genes in mouse liver ( n = 6). ( F ) Expression levels of gluconeogenic genes and their regulator Pgc1α in mouse liver ( n = 6). ( G ) Expression level of the Mkp-3 gene in mouse liver ( n = 6). ( H , I ) Protein levels of p-PERK and MKP-3 in mouse liver ( n = 6). Data are presented as mean ± standard error. * p < 0.05, ** p < 0.01, ns indicates no significant difference.

Article Snippet: Briefly, Mkp-3 loxp/loxp mice (S-CKO-13779, Cyagen Biosciences, Guangzhou, China) were mated with albumin-Cre mice (Jackson Laboratory, Bar Harbor, ME, USA) to generate Mkp-3 LKO ( Mkp-3 loxp/loxp , Cre + ) mice.

Techniques: Expressing, Injection, Saline, Marker

Journal: International Journal of Molecular Sciences

Article Title: MAPK Phosphatase-3 Mediates Chronic Endoplasmic Reticulum Stress Promoting Hepatic Gluconeogenesis

doi: 10.3390/ijms27062874

Figure Lengend Snippet: Prolonged excess energy intake induced hepatic ER stress and promoted gluconeogenesis and Mkp-3 expression. ( A ) Mice were fed with a high-fat diet (HFD) or standard chow diet for 28 weeks. ( B ) Changes in body weight during the treatment ( n = 12). ( C ) Fasting blood glucose concentration in mice ( n = 12). ( D ) Weights of liver, gonadal fat (GF) and subcutaneous fat (SCF) in the mice ( n = 12). ( E ) Serum concentrations of non-esterified fatty acids (NEFA), total cholesterol (TC), and triglyceride (TAG) in the mice ( n = 12). ( F ) Expression levels of Grp78 and Chop in mouse liver ( n = 12). ( G ) Expression levels of Pepck , G6pc and Pgc1α in mouse liver ( n = 12). ( H ) Expression levels of Mkp-3 in mouse liver ( n = 12). ( I , J ) Protein levels of p-PERK, PERK and MKP-3 in mouse liver ( n = 6). Data are presented as mean ± standard error. * p < 0.05, ** p < 0.01, *** p < 0.001, ns indicates no significant difference.

Article Snippet: Briefly, Mkp-3 loxp/loxp mice (S-CKO-13779, Cyagen Biosciences, Guangzhou, China) were mated with albumin-Cre mice (Jackson Laboratory, Bar Harbor, ME, USA) to generate Mkp-3 LKO ( Mkp-3 loxp/loxp , Cre + ) mice.

Techniques: Expressing, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: MAPK Phosphatase-3 Mediates Chronic Endoplasmic Reticulum Stress Promoting Hepatic Gluconeogenesis

doi: 10.3390/ijms27062874

Figure Lengend Snippet: Role of MKP-3 in chronic ER stress promoting hepatic gluconeogenesis. ( A ) Wild-type (WT) and liver-specific Mkp-3 knockout ( Mkp-3 LKO) mice were fed with a HFD or standard chow diet for 14 weeks. ( B ) Body weight at harvest time ( n = 5). ( C ) Liver weight (n = 5). ( D ) Gonadal fat weight ( n = 5). ( E ) Fasting blood glucose concentration ( n = 5). ( F ) Expression levels of Grp78 and Chop in mouse liver ( n = 5). ( G ) Expression levels of Pepck , G6pc , and Pgc1α in mouse liver ( n = 5). Data are presented as mean ± standard error. * p < 0.05, ** p < 0.01, *** p < 0.001, ns indicates no significant difference.

Article Snippet: Briefly, Mkp-3 loxp/loxp mice (S-CKO-13779, Cyagen Biosciences, Guangzhou, China) were mated with albumin-Cre mice (Jackson Laboratory, Bar Harbor, ME, USA) to generate Mkp-3 LKO ( Mkp-3 loxp/loxp , Cre + ) mice.

Techniques: Knock-Out, Concentration Assay, Expressing

Journal: International Journal of Molecular Sciences

Article Title: MAPK Phosphatase-3 Mediates Chronic Endoplasmic Reticulum Stress Promoting Hepatic Gluconeogenesis

doi: 10.3390/ijms27062874

Figure Lengend Snippet: Chronic ER stress promoted Mkp-3 expression via the PERK pathway. ( A ) Primary mouse hepatocytes were treated with a PERK agonist CCT020312 at 0.4 μM, gene expression level of Mkp-3 was then measured ( n = 3). ( B ) Primary mouse hepatocytes were co-treated with Tg and a PERK inhibitor GSK2656157 at 0.04 μM, gene expression level of Mkp-3 was then measured ( n = 3). ( C , D ) Primary mouse hepatocytes were treated with an IRE1 inhibitor 4µ8c, protein level of XBP1 ( C ) and gene expression level of Mkp-3 were measured ( n = 3). Data are presented as mean ± standard error. ** p < 0.01, *** p < 0.001, ns indicates no significant difference.

Article Snippet: Briefly, Mkp-3 loxp/loxp mice (S-CKO-13779, Cyagen Biosciences, Guangzhou, China) were mated with albumin-Cre mice (Jackson Laboratory, Bar Harbor, ME, USA) to generate Mkp-3 LKO ( Mkp-3 loxp/loxp , Cre + ) mice.

Techniques: Expressing, Gene Expression